Gene:  Eif4g2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F) which is a cap binding protein complex that consists of three subunits: eIF4A eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G in addition contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation this gene product functions as a general repressor of translation by forming translationally inactive complexes. Transgene expression of the apolipoprotein B mRNA-editing enzyme (APOBEC-1) causes extensive editing of this mRNA which could contribute to the potent oncogenesis induced by overexpression of APOBEC-1. In vitro and in vivo studies in human indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. This also appears to be true for mouse. Two alternatively spliced transcript variants that encode different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCED0045449
Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0053875
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0016742
List Price:    $125.00
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PrimePCR™ Probe Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCEP0053875
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5065658
Info:   EG; Same primer pair as used in qPCR assay qMmuCED0045449; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5080240
Info:   EG; Same primer pair as used in qPCR assay qMmuCID0016742; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5094946
Info:   FAM; Same primer pair and probe as used in qPCR assay qMmuCEP0053875; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Eif4g2, Mouse
Eukaryotic translation initiation factor 4, gamma 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5094947
Info:   HEX; Same primer pair and probe as used in qPCR assay qMmuCEP0053875; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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