Gene:  EIF4G2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F) which is a cap binding protein complex that consists of three subunits: eIF4A eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G in addition contains a binding site for eIF4E at the N-terminus. Unlike eIF4G which supports cap-dependent and independent translation this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043310
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050572
List Price:    $138.00
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PrimePCR™ SYBR® Green Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0020242
List Price:    $138.00
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PrimePCR™ Probe Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050572
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008092
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043310; exonic
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5023517
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0020242; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034496
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050572; exonic
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: EIF4G2, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034497
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050572; exonic
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:EIF4G2 p.E736G, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505664
Info:   73nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:EIF4G2 WT for p.E736G, Human
eukaryotic translation initiation factor 4 gamma, 2

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505665
Info:   73nt amplicon; exonic; in silico
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