Gene:  Egln1, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a component of a transcriptional complex that plays a central role in mammalian oxygen homeostasis. Hypoxia reduces the activity of prolyl hyxroxylases that hydroxylate specific proline residues of the hypoxia-inducible factor-1a (Hif1a). In the absence of hydroxylation, the Hif1a transcription factor accumulates and activates transcription of hypoxia-responsive target genes. This gene encodes one of the three known Hif-interacting 2-oxoglutarate/iron-dependent prolyl-hydroxylases (HIF-PHDs) in rat. Targeted disruption of this gene in mice produced embryonic lethality between embryonic day 12.5 and day 14.5. Based on the transcript data currently available for rat, this Reference Sequence is believed to contain the complete coding region for this gene. However, when compared to its mouse and human orthologs, it has a shorter 5' coding region and an incomplete N-terminus zf-MYND domain. This locus currently has limited transcript data and aligns to an unfinished region of the rat reference genome assembly. It is therefore uncertain whether its coding region can be extended at the 5' end to encode a complete zf-MYND domain, whether no further changes need to be made to its coding region, or whether it is a transcribed pseudogene that does not encode a functional protein. As more transcript and experimental data become available, the coding status of this locus may change. [provided by RefSeq, Jul 2008]

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PrimePCR™ SYBR® Green Assay: Egln1, Rat
Egl nine homolog 1

Assay Type: SYBR® Green
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qRnoCID0007894
List Price:    $130.00
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PrimePCR™ Probe Assay: Egln1, Rat
Egl nine homolog 1

Assay Type: Probe
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qRnoCIP0029725
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Egln1, Rat
Egl nine homolog 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dRnoEG5139465
Info:   EG; Same primer pair as used in qPCR assay qRnoCID0007894; intron_spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Egln1, Rat
Egl nine homolog 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5174908
Info:   FAM; Same primer pair and probe as used in qPCR assay qRnoCIP0029725; intron_spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Egln1, Rat
Egl nine homolog 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5174909
Info:   HEX; Same primer pair and probe as used in qPCR assay qRnoCIP0029725; intron_spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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