Gene:  ECH1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species particularly within a conserved domain characteristic of these proteins. The encoded protein which contains a C-terminal peroxisomal targeting sequence localizes to the peroxisome. The rat ortholog which localizes to the matrix of both the peroxisome and mitochondria can isomerize 3-trans5-cis-dienoyl-CoA to 2-trans4-trans-dienoyl-CoA indicating that it is a delta35-delta24-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: ECH1, Human
enoyl CoA hydratase 1, peroxisomal

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011498
Info:   Same primer pair as used in probe assay qHsaCIP0028047
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PrimePCR™ Probe Assay: ECH1, Human
enoyl CoA hydratase 1, peroxisomal

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028047
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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