Gene:  DUT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA which induces extensive excision repair mediated by uracil glycosylase. This repair process resulting in the removal and reincorporation of dUTP is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002835
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044240
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051502
List Price:    $130.00
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Add to Custom Plate
PrimePCR™ Probe Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051502
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001461
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002835; Exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008914
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044240; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036140
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051502; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DUT, Human
deoxyuridine triphosphatase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5036141
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051502; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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