Gene:  DUSP4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1 ERK2 and JNK is expressed in a variety of tissues and is localized in the nucleus. Two alternatively spliced transcript variants encoding distinct isoforms have been observed for this gene. In addition multiple polyadenylation sites have been reported. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: DUSP4, Human
dual specificity phosphatase 4

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0034774
Info:   Same primer pair as used in probe assay qHsaCEP0035599
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PrimePCR™ Probe Assay: DUSP4, Human
dual specificity phosphatase 4

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0035599
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DUSP4, Human
dual specificity phosphatase 4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005524
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0034774; Exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: DUSP4, Human
dual specificity phosphatase 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029360
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0035599; Exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: DUSP4, Human
dual specificity phosphatase 4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029361
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0035599; Exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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