Gene:  DNMT3L, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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CpG methylation is an epigenetic modification that is important for embryonic development imprinting and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: DNMT3L, Human
DNA (cytosine-5-)-methyltransferase 3-like

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0018059
Info:   Same primer pair as used in probe assay qHsaCIP0031625
List Price:    $125.00
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PrimePCR™ Probe Assay: DNMT3L, Human
DNA (cytosine-5-)-methyltransferase 3-like

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031625
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DNMT3L, Human
DNA (cytosine-5-)-methyltransferase 3-like

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022980
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0018059; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DNMT3L, Human
DNA (cytosine-5-)-methyltransferase 3-like

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056368
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031625; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DNMT3L, Human
DNA (cytosine-5-)-methyltransferase 3-like

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056369
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031625; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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