Gene:  DNMT3A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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CpG methylation is an epigenetic modification that is important for embryonic development imprinting and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: DNMT3A, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010863
Info:   Same primer pair as used in probe assay qHsaCIP0027759
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PrimePCR™ Probe Assay: DNMT3A, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027759
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PrimePCR™ ddPCR™ Mutation Assay: DNMT3A p.R882H, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000089
Info:   57nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: DNMT3A WT for p.R882H, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000090
Info:   57nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.Y735C, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501038
Info:   62nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.Y735C, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501039
Info:   62nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R792H, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501256
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R792H, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501257
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.N717S, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501374
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.N717S, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501375
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R882P, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501764
Info:   57nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R882P, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501765
Info:   57nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R598*, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501956
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R598*, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501957
Info:   65nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R749C, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501996
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R749C, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501997
Info:   63nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R771Q, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503256
Info:   66nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R771Q, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503257
Info:   66nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.S714C, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503498
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.S714C, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503499
Info:   65nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R635W, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503628
Info:   76nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R635W, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503629
Info:   76nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.R882S, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504484
Info:   57nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.R882S, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504485
Info:   57nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A p.P904L, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504704
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:DNMT3A WT for p.P904L, Human
DNA (cytosine-5-)-methyltransferase 3 alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504705
Info:   68nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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