Gene:  DLAT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene dihydrolipoamide acetyltransferase accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq Oct 2009]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045488
Info:   Same primer pair as used in probe assay qHsaCEP0052749
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0012546
Info:   Recommended - best efficiency
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052749
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018714
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0012546; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5189157
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0045488; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192005
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052749; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DLAT, Human
dihydrolipoamide S-acetyltransferase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5192006
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052749; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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