Gene:  Dio1, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene belongs to the iodothyronine deiodinase family. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). This protein contains a selenocysteine (Sec) residue which is necessary for maximal enzyme activity. The selenocysteine is encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Impaired expression of this gene results in hypothyroidism which causes severe defects in the development of many different tissues. [provided by RefSeq Jul 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCED0049506
Info:   Recommended - best coverage; Same primer pair as used in probe assay qMmuCEP0057932
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0014619
List Price:    $125.00
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PrimePCR™ Probe Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qMmuCEP0057932
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5069446
Info:   EG; Same primer pair as used in qPCR assay qMmuCED0049506; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5078930
Info:   EG; Same primer pair as used in qPCR assay qMmuCID0014619; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5102522
Info:   FAM; Same primer pair and probe as used in qPCR assay qMmuCEP0057932; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Dio1, Mouse
Deiodinase, iodothyronine, type I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5102523
Info:   HEX; Same primer pair and probe as used in qPCR assay qMmuCEP0057932; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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