Gene:  DIO1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 33'5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some but not all isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure the sec insertion sequence (SECIS) which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037912
Info:   Same primer pair as used in probe assay qHsaCEP0040635
List Price:    $125.00
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PrimePCR™ Probe Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040635
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006316
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037912; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5030944
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040635; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: DIO1, Human
deiodinase, iodothyronine, type I

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5030945
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040635; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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