Gene:  DHH, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq May 2010]

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PrimePCR™ SYBR® Green Assay: DHH, Human
desert hedgehog

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002676
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PrimePCR™ SYBR® Green Assay: DHH, Human
desert hedgehog

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044918
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0052180
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PrimePCR™ Probe Assay: DHH, Human
desert hedgehog

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052180
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DHH, Human
desert hedgehog

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001333
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002676; Exonic
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: DHH, Human
desert hedgehog

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009518
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0044918; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: DHH, Human
desert hedgehog

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5037348
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052180; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: DHH, Human
desert hedgehog

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5037349
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052180; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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