Gene:  DFFB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: DFFB, Human
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046647
Info:   Same primer pair as used in probe assay qHsaCEP0053908
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PrimePCR™ SYBR® Green Assay: DFFB, Human
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010170
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: DFFB, Human
DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0053908
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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