Gene:  CYBB, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CYBB, Human
cytochrome b-245, beta polypeptide

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015139
Info:   Same primer pair as used in probe assay qHsaCIP0029732
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PrimePCR™ Probe Assay: CYBB, Human
cytochrome b-245, beta polypeptide

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0029732
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CYBB, Human
cytochrome b-245, beta polypeptide

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5020576
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0015139; Intron-spanning
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PrimePCR™ ddPCR™ Expression Probe Assay: CYBB, Human
cytochrome b-245, beta polypeptide

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052846
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0029732; Intron-spanning
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: CYBB, Human
cytochrome b-245, beta polypeptide

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5052847
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0029732; Intron-spanning
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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