Gene:  CYB5R3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes cytochrome b5 reductase which includes a membrane-bound form in somatic cells (anchored in the endoplasmic reticulum mitochondrial and other membranes) and a soluble form in erythrocytes. The membrane-bound form exists mainly on the cytoplasmic side of the endoplasmic reticulum and functions in desaturation and elongation of fatty acids in cholesterol biosynthesis and in drug metabolism. The erythrocyte form is located in a soluble fraction of circulating erythrocytes and is involved in methemoglobin reduction. The membrane-bound form has both membrane-binding and catalytic domains while the soluble form has only the catalytic domain. Alternate splicing results in multiple transcript variants. Mutations in this gene cause methemoglobinemias. [provided by RefSeq Jan 2010]

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PrimePCR™ SYBR® Green Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042758
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0050020
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009335
List Price:    $130.00
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PrimePCR™ Probe Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050020
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007606
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0042758; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016314
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009335; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033524
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050020; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CYB5R3, Human
cytochrome b5 reductase 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5033525
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050020; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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