Gene:  CRYBA1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Crystallins are separated into two classes: taxon-specific or enzyme and ubiquitous. The latter class constitutes the major proteins of vertebrate eye lens and maintains the transparency and refractive index of the lens. Since lens central fiber cells lose their nuclei during development these crystallins are made and then retained throughout life making them extremely stable proteins. Mammalian lens crystallins are divided into alpha beta and gamma families; beta and gamma crystallins are also considered as a superfamily. Alpha and beta families are further divided into acidic and basic groups. Seven protein regions exist in crystallins: four homologous motifs a connecting peptide and N- and C-terminal extensions. Beta-crystallins the most heterogeneous differ by the presence of the C-terminal extension (present in the basic group none in the acidic group). Beta-crystallins form aggregates of different sizes and are able to self-associate to form dimers or to form heterodimers with other beta-crystallins. This gene a beta acidic group member encodes two proteins (crystallin beta A3 and crystallin beta A1) from a single mRNA the latter protein is 17 aa shorter than crystallin beta A3 and is generated by use of an alternate translation initiation site. Deletion of exons 3 and 4 causes the autosomal dominant disease 'zonular cataract with sutural opacities'. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CRYBA1, Human
crystallin, beta A1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009686
Info:   Same primer pair as used in probe assay qHsaCIP0027289
List Price:    $125.00
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PrimePCR™ Probe Assay: CRYBA1, Human
crystallin, beta A1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027289
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CRYBA1, Human
crystallin, beta A1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016566
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0009686; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CRYBA1, Human
crystallin, beta A1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048730
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027289; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CRYBA1, Human
crystallin, beta A1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048731
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027289; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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