Gene:  CPSF4, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4 an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular and not viral mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CPSF4, Human
cleavage and polyadenylation specific factor 4, 30kDa

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0001334
Info:   Same primer pair as used in probe assay qHsaCEP0024027
List Price:    $125.00
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PrimePCR™ Probe Assay: CPSF4, Human
cleavage and polyadenylation specific factor 4, 30kDa

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0024027
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CPSF4, Human
cleavage and polyadenylation specific factor 4, 30kDa

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5000280
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0001334; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CPSF4, Human
cleavage and polyadenylation specific factor 4, 30kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5025514
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0024027; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CPSF4, Human
cleavage and polyadenylation specific factor 4, 30kDa

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5025515
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0024027; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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