Gene:  COL4A3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Type IV collagen the major structural component of basement membranes is a multimeric protein composed of 3 alpha subunits. These subunits are encoded by 6 different genes alpha 1 through alpha 6 each of which can form a triple helix structure with 2 other subunits to form type IV collagen. This gene encodes alpha 3. In the Goodpasture syndrome autoantibodies bind to the collagen molecules in the basement membranes of alveoli and glomeruli. The epitopes that elicit these autoantibodies are localized largely to the non-collagenous C-terminal domain of the protein. A specific kinase phosphorylates amino acids in this same C-terminal region and the expression of this kinase is upregulated during pathogenesis. This gene is also linked to an autosomal recessive form of Alport syndrome. The mutations contributing to this syndrome are also located within the exons that encode this C-terminal region. Like the other members of the type IV collagen gene family this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq Jun 2010]

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PrimePCR™ SYBR® Green Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0046600
Info:   Same primer pair as used in probe assay qHsaCEP0053861
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013308
Info:   Recommended - best coverage
List Price:    $125.00
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PrimePCR™ Probe Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0053861
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5011030
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0046600; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019241
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013308; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040370
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0053861; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: COL4A3, Human
collagen, type IV, alpha 3 (Goodpasture antigen)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5040371
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0053861; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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