Gene:  CLCN3, Human

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This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq Aug 2011]

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PrimePCR™ SYBR® Green Assay: CLCN3, Human
chloride channel 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043281
Info:   Same primer pair as used in probe assay qHsaCEP0050543
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: CLCN3, Human
chloride channel 3

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0007179
Info:   Recommended - best coverage
List Price:    $125.00
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PrimePCR™ Probe Assay: CLCN3, Human
chloride channel 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050543
List Price:    $355.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CLCN3, Human
chloride channel 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008066
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043281; exonic
List Price:    $120.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CLCN3, Human
chloride channel 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5014880
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0007179; Intron-spanning
List Price:    $120.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CLCN3, Human
chloride channel 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034444
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050543; exonic
 
Fluorophore:   FAM
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CLCN3, Human
chloride channel 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034445
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050543; exonic
 
Fluorophore:   HEX
List Price:    $180.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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