Gene:  CHRND, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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The acetylcholine receptor of muscle has 5 subunits of 4 different types: 2 alpha and 1 each of beta gamma and delta subunits. After acetylcholine binding the receptor undergoes an extensive conformation change that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Defects in this gene are a cause of multiple pterygium syndrome lethal type (MUPSL) congenital myasthenic syndrome slow-channel type (SCCMS) and congenital myasthenic syndrome fast-channel type (FCCMS). Several transcript variants some protein-coding and some not have been found for this gene. [provided by RefSeq Feb 2012]

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PrimePCR™ SYBR® Green Assay: CHRND, Human
cholinergic receptor, nicotinic, delta

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038546
Info:   Same primer pair as used in probe assay qHsaCEP0041268
List Price:    $130.00
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PrimePCR™ Probe Assay: CHRND, Human
cholinergic receptor, nicotinic, delta

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0041268
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CHRND, Human
cholinergic receptor, nicotinic, delta

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006573
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0038546; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CHRND, Human
cholinergic receptor, nicotinic, delta

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5031458
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0041268; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CHRND, Human
cholinergic receptor, nicotinic, delta

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5031459
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0041268; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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