Gene:  CHRNB3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if like muscle alpha-1 (MIM 100690) they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains a variable cytoplasmic loop a fourth conserved transmembrane domain and a short C-terminal extracellular region.[supplied by OMIM Apr 2010]

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PrimePCR™ SYBR® Green Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0034077
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0045368
Info:   Same primer pair as used in probe assay qHsaCEP0052629
List Price:    $125.00
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Add to Custom Plate
PrimePCR™ Probe Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0052629
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005250
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0034077; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5009917
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0045368; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5038144
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0052629; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CHRNB3, Human
cholinergic receptor, nicotinic, beta 3

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5038145
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0052629; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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