Gene:  CHRNA2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels formed by a pentameric arrangement of alpha and beta subunits to create distinct muscle and neuronal receptors. Neuronal receptors are found throughout the peripheral and central nervous system where they are involved in fast synaptic transmission. This gene encodes an alpha subunit that is widely expressed in the brain. The proposed structure for nAChR subunits is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains a variable cytoplasmic loop a fourth conserved transmembrane domain and a short C-terminal extracellular region. Mutations in this gene cause autosomal dominant nocturnal frontal lobe epilepsy type 4. Single nucleotide polymorphisms (SNPs) in this gene have been associated with nicotine dependence. [provided by RefSeq Nov 2009]

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PrimePCR™ SYBR® Green Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043885
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051147
List Price:    $125.00
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PrimePCR™ SYBR® Green Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0022248
List Price:    $125.00
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PrimePCR™ Probe Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051147
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5008594
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043885; exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5024348
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0022248; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Copy Number Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000284
Info:   FAM: 78nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2507002
Info:   HEX; 78nt amplicon; exonic; HEX version of CHRNA2 (dHsaCP1000284)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035500
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0051147; exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CHRNA2, Human
cholinergic receptor, nicotinic, alpha 2 (neuronal)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5035501
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0051147; exonic
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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