Gene:  CHAT, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene and some of these variants have been shown to encode more than one isoform. [provided by RefSeq May 2010]

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PrimePCR™ SYBR® Green Assay: CHAT, Human
choline O-acetyltransferase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042296
Info:   Recommended - best efficiency; Same primer pair as used in probe assay qHsaCEP0049559
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PrimePCR™ SYBR® Green Assay: CHAT, Human
choline O-acetyltransferase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0009484
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PrimePCR™ Probe Assay: CHAT, Human
choline O-acetyltransferase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049559
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PrimePCR™ ddPCR™ Copy Number Assay: CHAT, Human
choline O-acetyltransferase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP1000387
Info:   68nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CHAT p.R323H, Human
choline O-acetyltransferase

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505414
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CHAT WT for p.R323H, Human
choline O-acetyltransferase

Assay Type: Probe
Application: Mutation detection (In Silico design)
Unique Assay ID: dHsaIS2505415
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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