Gene:  CH25H, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This is an intronless gene that is involved in cholesterol and lipid metabolism. The encoded protein is a membrane protein and contains clusters of histidine residues essential for catalytic activity. Unlike most other sterol hydroxylases this enzyme is a member of a small family of enzymes that utilize diiron cofactors to catalyze the hydroxylation of hydrophobic substrates. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CH25H, Human
cholesterol 25-hydroxylase

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0018948
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PrimePCR™ SYBR® Green Assay: CH25H, Human
cholesterol 25-hydroxylase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057327
Info:   Same primer pair as used in probe assay qHsaCEP0058432
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PrimePCR™ Probe Assay: CH25H, Human
cholesterol 25-hydroxylase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058432
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PrimePCR™ ddPCR™ Copy Number Assay: CH25H, Human
cholesterol 25-hydroxylase

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500377
Info:   68nt amplicon; exonic
 
Fluorophore:   FAM
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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