Gene:  CDC27, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27 and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC) which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat) a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2 p55CDC and BUBR1 and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: CDC27, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0022949
Info:   Recommended - best efficiency
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PrimePCR™ SYBR® Green Assay: CDC27, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0041891
Info:   Same primer pair as used in probe assay qHsaCEP0049154
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PrimePCR™ Probe Assay: CDC27, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049154
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 p.N453S, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500942
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 WT for p.N453S, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500943
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 p.A274D, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501308
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 WT for p.A274D, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501309
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 p.D549E, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504346
Info:   107nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 WT for p.D549E, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504347
Info:   107nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 p.I487V, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504754
Info:   120nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 WT for p.I487V, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504755
Info:   120nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 p.Q257Q, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505048
Info:   120nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 WT for p.Q257Q, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505049
Info:   120nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 p.T167T, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505748
Info:   117nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:CDC27 WT for p.T167T, Human
cell division cycle 27 homolog (S. cerevisiae)

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505749
Info:   117nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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