Gene:  CAPN10, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Calpains represent a ubiquitous well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I the N-terminal regulatory domain that is processed upon calpain activation; domain II the protease domain; domain III a linker domain of unknown function; and domain IV the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq Sep 2010]

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PrimePCR™ SYBR® Green Assay: CAPN10, Human
calpain 10

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005041
Info:   Same primer pair as used in probe assay qHsaCEP0025255
List Price:    $125.00
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PrimePCR™ Probe Assay: CAPN10, Human
calpain 10

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0025255
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: CAPN10, Human
calpain 10

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5003115
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0005041; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CAPN10, Human
calpain 10

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5027314
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0025255; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: CAPN10, Human
calpain 10

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5027315
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0025255; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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