Gene:  C5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is the fifth component of complement which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide C5a which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component and this complex is the basis for formation of the membrane attack complex which includes additional complement components. Mutations in this gene cause complement component 5 deficiency a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to a susceptibility to liver fibrosis and to rheumatoid arthritis. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: C5, Human
complement component 5

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017789
Info:   Same primer pair as used in probe assay qHsaCIP0031459
List Price:    $130.00
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PrimePCR™ Probe Assay: C5, Human
complement component 5

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0031459
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: C5, Human
complement component 5

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022777
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0017789; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: C5, Human
complement component 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056082
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0031459; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: C5, Human
complement component 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5056083
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0031459; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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