Gene:  BYSL, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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Bystin is expressed as a 2-kb major transcript and a 3.6-kb minor transcript in SNG-M cells and in human trophoblastic teratocarcinoma HT-H cells. Protein binding assays determined that bystin binds directly to trophinin and tastin and that binding is enhanced when cytokeratins 8 and 18 are present. Immunocytochemistry of HT-H cells showed that bystin colocalizes with trophinin tastin and the cytokeratins suggesting that these molecules form a complex in trophectoderm cells at the time of implantation. Using immunohistochemistry it was determined that trophinin and bystin are found in the placenta from the sixth week of pregnancy. Both proteins were localized in the cytoplasm of the syncytiotrophoblast in the chorionic villi and in endometrial decidual cells at the uteroplacental interface. After week 10 the levels of trophinin tastin and bystin decreased and then disappeared from placental villi. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: BYSL, Human
bystin-like

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011721
Info:   Same primer pair as used in probe assay qHsaCIP0028150
List Price:    $130.00
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PrimePCR™ Probe Assay: BYSL, Human
bystin-like

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028150
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: BYSL, Human
bystin-like

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018091
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0011721; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BYSL, Human
bystin-like

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050202
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028150; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BYSL, Human
bystin-like

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050203
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028150; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
Quantity:  
 
    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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