Gene:  BRAF, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway which affects cell division differentiation and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome a disease characterized by heart defects mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers including non-Hodgkin lymphoma colorectal cancer malignant melanoma thyroid carcinoma non-small cell lung carcinoma and adenocarcinoma of lung. A pseudogene which is located on chromosome X has been identified for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0023755
Info:   Same primer pair as used in probe assay qHsaCIP0033856
List Price:    $125.00
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PrimePCR™ Probe Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033856
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5024824
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0023755; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000027
Info:   91nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000028
Info:   91nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600K, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000035
Info:   91nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600K, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000036
Info:   91nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000037
Info:   91nt amplicon; exonic
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000038
Info:   91nt amplicon; exonic
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Copy Number Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500366
Info:   FAM; 66nt amplicon; exonic; FAM version of BRAF (dHsaCP2506666)
 
Fluorophore:   FAM
List Price:    $230.00
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PrimePCR™ ddPCR™ Copy Number Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506666
Info:   HEX; 66nt amplicon; exonic; HEX version of BRAF (dHsaCP2500366)
 
Fluorophore:   HEX
List Price:    $230.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.G469A, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506932
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
List Price:    $380.00
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.G469A, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2506933
Info:   70nt amplicon; crosses exon-intron junction
 
Fluorophore:   HEX
List Price:    $380.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5058344
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0033856; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5058345
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0033856; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.N581S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501810
Info:   76nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.N581S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501811
Info:   76nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.D594G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502368
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.D594G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502369
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600M, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502624
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600M, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502625
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G464E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503360
Info:   100nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G464E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503361
Info:   100nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $315.00
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.K601E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504434
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.K601E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504435
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G466R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504624
Info:   100nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G466R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504625
Info:   100nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504938
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504939
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.L597R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505092
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.L597R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505093
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.L597Q, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505346
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.L597Q, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505347
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505652
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505653
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.T599I, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505872
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.T599I, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505873
Info:   91nt amplicon; exonic; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF p.D594N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505962
Info:   80nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.D594N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505963
Info:   80nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G466E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506036
Info:   80nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   FAM
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G466E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506037
Info:   80nt amplicon; crosses exon-intron junction; in silico
 
Fluorophore:   HEX
List Price:    $315.00
Your Price:   Log In
Quantity:  
 
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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