Gene:  BRAF, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway which affects cell division differentiation and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome a disease characterized by heart defects mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers including non-Hodgkin lymphoma colorectal cancer malignant melanoma thyroid carcinoma non-small cell lung carcinoma and adenocarcinoma of lung. A pseudogene which is located on chromosome X has been identified for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0023755
Info:   Same primer pair as used in probe assay qHsaCIP0033856
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PrimePCR™ Probe Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033856
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000027
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000028
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600K, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000035
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600K, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000036
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000037
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000038
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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