Gene:  BRAF, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway which affects cell division differentiation and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome a disease characterized by heart defects mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers including non-Hodgkin lymphoma colorectal cancer malignant melanoma thyroid carcinoma non-small cell lung carcinoma and adenocarcinoma of lung. A pseudogene which is located on chromosome X has been identified for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0023755
Info:   Same primer pair as used in probe assay qHsaCIP0033856
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PrimePCR™ Probe Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033856
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000027
Info:   91nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000028
Info:   91nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600K, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000035
Info:   91nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600K, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000036
Info:   91nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay: BRAF p.V600R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000037
Info:   91nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay: BRAF WT for p.V600R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Mutation Detection
Unique Assay ID: dHsaCP2000038
Info:   91nt amplicon; exonic
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Copy Number Assay: BRAF, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500366
Info:   66nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600A, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500742
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600A, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500743
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.F595L, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500846
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.F595L, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500847
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G466V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500966
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G466V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2500967
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.S637P, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501008
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.S637P, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501009
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.F583F, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501380
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.F583F, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501381
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G464V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501394
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G464V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501395
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.N581S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501810
Info:   76nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.N581S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2501811
Info:   76nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G596G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502150
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G596G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502151
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.S605G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502224
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.S605G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502225
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600L, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502302
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600L, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502303
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.D594G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502368
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.D594G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502369
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.Y472S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502608
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.Y472S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502609
Info:   70nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600M, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502624
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600M, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502625
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.R603*, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502774
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.R603*, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502775
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.S614P, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502880
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.S614P, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502881
Info:   69nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.A598V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502960
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.A598V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2502961
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G464E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503360
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G464E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503361
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G596R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503636
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G596R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503637
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.L597V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503860
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.L597V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503861
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.K601N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503902
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.K601N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503903
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.K601E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504434
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.K601E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504435
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.E586E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504526
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.E586E, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504527
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G466R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504624
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G466R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504625
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.I592M, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504778
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.I592M, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504779
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.L618W, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504788
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.L618W, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504789
Info:   68nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504938
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504939
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.L597R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505092
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.L597R, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505093
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.L597Q, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505346
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.L597Q, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505347
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.K601N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505502
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.K601N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505503
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.F595S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505544
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.F595S, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505545
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.V600V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505652
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.V600V, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505653
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.G460G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505792
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.G460G, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505793
Info:   100nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.T599I, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505872
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:BRAF WT for p.T599I, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505873
Info:   91nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:BRAF p.D594N, Human
v-raf murine sarcoma viral oncogene homolog B1

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505962
Info:   80nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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