Gene:  BOLA3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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This gene encodes a protein that plays an essential role in the production of iron-sulfur (Fe-S) clusters for the normal maturation of lipoate-containing 2-oxoacid dehydrogenases and for the assembly of the mitochondrial respiratory chain complexes. Mutation in this gene has been associated with multiple mitochondrial dysfunctions syndrome-2. Two alternatively spliced transcript variants encoding different isoforms with distinct subcellular localization have been reported for this gene (PMID:21944046). [provided by RefSeq Dec 2011]

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PrimePCR™ SYBR® Green Assay: BOLA3, Human
bolA homolog 3 (E. coli)

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0002816
Info:   Same primer pair as used in probe assay qHsaCEP0024514
List Price:    $125.00
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PrimePCR™ Probe Assay: BOLA3, Human
bolA homolog 3 (E. coli)

Assay Type: Probe
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0024514
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: BOLA3, Human
bolA homolog 3 (E. coli)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5001448
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0002816; Exonic
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BOLA3, Human
bolA homolog 3 (E. coli)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5026248
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0024514; Exonic
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: BOLA3, Human
bolA homolog 3 (E. coli)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5026249
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0024514; Exonic
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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