Gene:  AXIN1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain. The encoded protein interacts with adenomatosis polyposis coli catenin beta-1 glycogen synthase kinase 3 beta protein phosphate 2 and itself. This protein functions as a negative regulator of the wingless-type MMTV integration site family member 1 (WNT) signaling pathway and can induce apoptosis. The crystal structure of a portion of this protein alone and in a complex with other proteins has been resolved. Mutations in this gene have been associated with hepatocellular carcinoma hepatoblastomas ovarian endometriod adenocarcinomas and medullablastomas. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Dec 2010]

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PrimePCR™ SYBR® Green Assay: AXIN1, Human
axin 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010131
Info:   Same primer pair as used in probe assay qHsaCIP0027462
List Price:    $138.00
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PrimePCR™ Probe Assay: AXIN1, Human
axin 1

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0027462
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: AXIN1, Human
axin 1

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5016864
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0010131; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: AXIN1, Human
axin 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048998
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0027462; Intron-spanning
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: AXIN1, Human
axin 1

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5048999
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0027462; Intron-spanning
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:AXIN1 p.G572R, Human
axin 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503434
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AXIN1 WT for p.G572R, Human
axin 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503435
Info:   65nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AXIN1 p.T79I, Human
axin 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503684
Info:   63nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AXIN1 WT for p.T79I, Human
axin 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503685
Info:   63nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AXIN1 p.A76T, Human
axin 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504288
Info:   63nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AXIN1 WT for p.A76T, Human
axin 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504289
Info:   63nt amplicon; exonic; in silico
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