Gene:  ATR, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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The protein encoded by this gene belongs the PI3/PI4-kinase family and is most closely related to ATM a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3 a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1 checkpoint proteins RAD17 and RAD9 as well as tumor suppressor protein BRCA1. Mutations of this gene are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported however its full length nature is not known. Transcript variants utilizing alternative polyA sites exist. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ATR, Human
ataxia telangiectasia and Rad3 related

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0022638
Info:   Same primer pair as used in probe assay qHsaCIP0033514
List Price:    $125.00
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PrimePCR™ Probe Assay: ATR, Human
ataxia telangiectasia and Rad3 related

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033514
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ATR, Human
ataxia telangiectasia and Rad3 related

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5024515
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0022638; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ATR, Human
ataxia telangiectasia and Rad3 related

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5058058
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0033514; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ATR, Human
ataxia telangiectasia and Rad3 related

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5058059
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0033514; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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