Gene:  ATP6V0A4, Human

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This gene encodes a component of vacuolar ATPase (V-ATPase) a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting zymogen activation receptor-mediated endocytosis and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits two G subunits plus the C D E F and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a c c' c'' and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043112
Info:   Same primer pair as used in probe assay qHsaCEP0050374
List Price:    $130.00
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PrimePCR™ SYBR® Green Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0018053
List Price:    $130.00
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PrimePCR™ Probe Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0050374
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5007926
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0043112; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5022975
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0018053; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034164
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0050374; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ATP6V0A4, Human
ATPase, H+ transporting, lysosomal V0 subunit a4

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5034165
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0050374; exonic
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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