Gene:  ASAP2, Human

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This gene encodes a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif followed by a pleckstrin homology (PH) domain an Arf-GAP domain an ankyrin homology region a proline-rich region and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition it functions as a substrate and downstream target for PYK2 and SRC a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Sep 2008]

Select a product type :   All   qPCR   ddPCR
PrimePCR™ SYBR® Green Assay: ASAP2, Human
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0011781
Info:   Same primer pair as used in probe assay qHsaCIP0028178
List Price:    $125.00
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PrimePCR™ Probe Assay: ASAP2, Human
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028178
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ASAP2, Human
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5018138
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0011781; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ASAP2, Human
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050250
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028178; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ASAP2, Human
ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5050251
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028178; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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