Gene:  ARID1A, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the SWI/SNF family whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ARID1A, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043754
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051016
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PrimePCR™ SYBR® Green Assay: ARID1A, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0006352
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PrimePCR™ Probe Assay: ARID1A, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051016
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PrimePCR™ ddPCR™ Copy Number Assay: ARID1A, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500360
Info:   63nt amplicon; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.R1722*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501494
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.R1722*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2501495
Info:   70nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.G2087R, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503482
Info:   66nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.G2087R, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503483
Info:   66nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.R1721*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503530
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.R1721*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503531
Info:   68nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.R1989*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505000
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.R1989*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505001
Info:   77nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.G414G, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505124
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.G414G, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505125
Info:   64nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.R1335*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505890
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.R1335*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505891
Info:   69nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A p.R1276*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506052
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ARID1A WT for p.R1276*, Human
AT rich interactive domain 1A (SWI-like)

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506053
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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