Gene:  APP, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Design and Validation of Real-Time PCR Primers
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This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: APP, Human
amyloid beta (A4) precursor protein

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0057336
Info:   Same primer pair as used in probe assay qHsaCEP0058441
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PrimePCR™ SYBR® Green Assay: APP, Human
amyloid beta (A4) precursor protein

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0010429
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PrimePCR™ Probe Assay: APP, Human
amyloid beta (A4) precursor protein

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0058441
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PrimePCR™ ddPCR™ Copy Number Assay: APP, Human
amyloid beta (A4) precursor protein

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2500358
Info:   66nt amplicon; crosses exon-intron junction
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APP p.F512F, Human
amyloid beta (A4) precursor protein

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503544
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APP WT for p.F512F, Human
amyloid beta (A4) precursor protein

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2503545
Info:   62nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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