Gene:  APAF1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a cytoplasmic protein that initiates apoptosis. This protein contains several copies of the WD-40 domain a caspase recruitment domain (CARD) and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein releasing its mature activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: APAF1, Human
apoptotic peptidase activating factor 1

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0043883
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051145
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PrimePCR™ SYBR® Green Assay: APAF1, Human
apoptotic peptidase activating factor 1

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0008058
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PrimePCR™ Probe Assay: APAF1, Human
apoptotic peptidase activating factor 1

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051145
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PrimePCR™ ddPCR™ Mutation Assay:APAF1 p.E146E, Human
apoptotic peptidase activating factor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503366
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APAF1 WT for p.E146E, Human
apoptotic peptidase activating factor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2503367
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:APAF1 p.K131R, Human
apoptotic peptidase activating factor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504894
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:APAF1 WT for p.K131R, Human
apoptotic peptidase activating factor 1

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504895
Info:   65nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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