Gene:  ANK1, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility activation proliferation contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1 the prototype of this family was first discovered in the erythrocytes but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq Dec 2008]

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PrimePCR™ SYBR® Green Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0037923
Info:   Same primer pair as used in probe assay qHsaCEP0040646
List Price:    $130.00
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PrimePCR™ Probe Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040646
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5006323
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0037923; exonic
List Price:    $130.00
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PrimePCR™ ddPCR™ Copy Number Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506264
Info:   Centromeric 8p Assay (8p11.21); FAM; 65nt amplicon; crosses exon-intron junction; FAM version of ANK1 (dHsaCP2506686)
 
Fluorophore:   FAM
List Price:    $240.00
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PrimePCR™ ddPCR™ Copy Number Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: Probe
Application: Copy Number Variation
Unique Assay ID: dHsaCP2506686
Info:   Centromeric 8p Assay (8p11.21); HEX; 65nt amplicon; crosses exon-intron junction; HEX version of ANK1 (dHsaCP2506264)
 
Fluorophore:   HEX
List Price:    $240.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5030958
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0040646; exonic
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ANK1, Human
ankyrin 1, erythrocytic

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5030959
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0040646; exonic
 
Fluorophore:   HEX
List Price:    $195.00
Your Price:   Log In
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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