Gene:  ANAPC5, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a tetratricopeptide repeat-containing component of the anaphase promoting complex/cyclosome (APC/C) a large E3 ubiquitin ligase that controls cell cycle progression by targeting a number of cell cycle regulators such as B-type cyclins for 26S proteasome-mediated degradation through ubiquitination. The encoded protein is required for the proper ubiquitination function of APC/C and for the interaction of APC/C with transcription coactivators. It also interacts with polyA binding protein and represses internal ribosome entry site-mediated translation. Multiple transcript variants encoding different isoforms have been found for this gene. These differences cause translation initiation at a downstream AUG and result in a shorter protein (isoform b) compared to isoform a. [provided by RefSeq Nov 2008]

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PrimePCR™ SYBR® Green Assay: ANAPC5, Human
anaphase promoting complex subunit 5

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0013270
Info:   Same primer pair as used in probe assay qHsaCIP0028802
List Price:    $130.00
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PrimePCR™ Probe Assay: ANAPC5, Human
anaphase promoting complex subunit 5

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0028802
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ANAPC5, Human
anaphase promoting complex subunit 5

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5019211
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0013270; Intron-spanning
List Price:    $130.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ANAPC5, Human
anaphase promoting complex subunit 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051302
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0028802; Intron-spanning
 
Fluorophore:   FAM
List Price:    $195.00
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PrimePCR™ ddPCR™ Expression Probe Assay: ANAPC5, Human
anaphase promoting complex subunit 5

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5051303
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0028802; Intron-spanning
 
Fluorophore:   HEX
List Price:    $195.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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