Gene:  AMPH, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq Nov 2010]

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PrimePCR™ SYBR® Green Assay: AMPH, Human
amphiphysin

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0042408
Info:   Same primer pair as used in probe assay qHsaCEP0049670
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PrimePCR™ SYBR® Green Assay: AMPH, Human
amphiphysin

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0017927
Info:   Recommended - best coverage
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PrimePCR™ Probe Assay: AMPH, Human
amphiphysin

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0049670
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PrimePCR™ ddPCR™ Mutation Assay:AMPH p.R292W, Human
amphiphysin

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504258
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:AMPH WT for p.R292W, Human
amphiphysin

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2504259
Info:   63nt amplicon; crosses exon-intron junction; in-silico designed
 
Fluorophore:   HEX
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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