Gene:  AGL, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-16-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: AGL, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0022499
Info:   Same primer pair as used in probe assay qHsaCIP0033451
List Price:    $138.00
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PrimePCR™ Probe Assay: AGL, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCIP0033451
List Price:    $202.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: AGL, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5024449
Info:   EG; Same primer pair as used in qPCR assay qHsaCID0022499; Intron-spanning
List Price:    $138.00
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PrimePCR™ ddPCR™ Expression Probe Assay: AGL, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057992
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCIP0033451; Intron-spanning
 
Fluorophore:   FAM
List Price:    $207.00
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PrimePCR™ ddPCR™ Expression Probe Assay: AGL, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5057993
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCIP0033451; Intron-spanning
 
Fluorophore:   HEX
List Price:    $207.00
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PrimePCR™ ddPCR™ Mutation Assay:AGL p.D489G, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502746
Info:   70nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AGL WT for p.D489G, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2502747
Info:   70nt amplicon; exonic; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AGL p.E773E, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506026
Info:   78nt amplicon; crosses exon-intron junction; in silico
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PrimePCR™ ddPCR™ Mutation Assay:AGL WT for p.E773E, Human
amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2506027
Info:   78nt amplicon; crosses exon-intron junction; in silico
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