Gene:  Add2, Mouse

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes the beta subunit of the adducin family. Adducins encoded by alpha beta and gamma genes are heteromeric proteins that crosslink actin filaments with spectrin at the cytoskeletal membrane. This protein primarily found in the brain and hematopoietic cells is regulated by phosphorylation and calmodulin interactions as it promotes spectrin assembly onto actin filaments bundles actin and caps barbed ends of actin filaments. In mouse deficiency of this gene can lead to mild hemolytic anemia and impaired synaptic plasticity. Mutations of this gene in mouse serve as a pathophysiological model for hereditary spherocytosis and hereditary elliptocytosis. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq Dec 2012]

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PrimePCR™ SYBR® Green Assay: Add2, Mouse
Adducin 2 (beta)

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCID0005262
Info:   Same primer pair as used in probe assay qMmuCIP0031537
List Price:    $125.00
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PrimePCR™ Probe Assay: Add2, Mouse
Adducin 2 (beta)

Assay Type: Probe
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qMmuCIP0031537
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Add2, Mouse
Adducin 2 (beta)

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dMmuEG5072788
Info:   EG; Same primer pair as used in qPCR assay qMmuCID0005262; Intron-spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Add2, Mouse
Adducin 2 (beta)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5115370
Info:   FAM; Same primer pair and probe as used in qPCR assay qMmuCIP0031537; Intron-spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Add2, Mouse
Adducin 2 (beta)

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dMmuCPE5115371
Info:   HEX; Same primer pair and probe as used in qPCR assay qMmuCIP0031537; Intron-spanning
 
Fluorophore:   HEX
List Price:    $190.00
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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