Gene:  ACTN2, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions where it is involved in binding actin to the membrane. In contrast skeletal cardiac and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies where they help anchor the myofibrillar actin filaments. This gene encodes a muscle-specific alpha actinin isoform that is expressed in both skeletal and cardiac muscles. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ACTN2, Human
actinin, alpha 2

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0036336
Info:   Same primer pair as used in probe assay qHsaCEP0039060
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PrimePCR™ Probe Assay: ACTN2, Human
actinin, alpha 2

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0039060
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: ACTN2, Human
actinin, alpha 2

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dHsaEG5005636
Info:   EG; Same primer pair as used in qPCR assay qHsaCED0036336; exonic
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PrimePCR™ ddPCR™ Expression Probe Assay: ACTN2, Human
actinin, alpha 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029584
Info:   FAM; Same primer pair and probe as used in qPCR assay qHsaCEP0039060; exonic
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Expression Probe Assay: ACTN2, Human
actinin, alpha 2

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dHsaCPE5029585
Info:   HEX; Same primer pair and probe as used in qPCR assay qHsaCEP0039060; exonic
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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