Gene:  ACSL3, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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The protein encoded by this gene is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. Although differing in substrate specificity subcellular localization and tissue distribution all isozymes of this family convert free long-chain fatty acids into fatty acyl-CoA esters and thereby play a key role in lipid biosynthesis and fatty acid degradation. This isozyme is highly expressed in brain and preferentially utilizes myristate arachidonate and eicosapentaenoate as substrates. The amino acid sequence of this isozyme is 92% identical to that of rat homolog. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ACSL3, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: SYBR® Green
Assay Design: Exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0005039
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PrimePCR™ SYBR® Green Assay: ACSL3, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044006
Info:   Same primer pair as used in probe assay qHsaCEP0051268
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PrimePCR™ Probe Assay: ACSL3, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051268
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PrimePCR™ ddPCR™ Mutation Assay:ACSL3 p.T188S, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504392
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ACSL3 WT for p.T188S, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2504393
Info:   77nt amplicon; crosses exon-intron junction; in-silico
 
Fluorophore:   HEX
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PrimePCR™ ddPCR™ Mutation Assay:ACSL3 p.V658A, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505660
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ACSL3 WT for p.V658A, Human
acyl-CoA synthetase long-chain family member 3

Assay Type: Probe
Application: Mutation Detection in silico design
Unique Assay ID: dHsaIS2505661
Info:   67nt amplicon; exonic; in-silico
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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