Gene:  Aco1, Rat

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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This gene encodes a member of the aconitase/IPM isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Depending on iron levels in the cytosol, the encoded protein can function as either an aconitase enzyme or as an mRNA binding protein. When cellular iron levels are high, the encoded protein functions as an aconitase, an essential enzyme in the TCA cycle that catalyzes the conversion of citrate to isocitrate. When cellular iron levels are low, the encoded protein regulates iron uptake and utilization by binding to iron-responsive elements in the untranslated regions of mRNAs for genes involved in iron metabolism [provided by RefSeq, Jan 2014]

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PrimePCR™ SYBR® Green Assay: Aco1, Rat
Cytoplasmic aconitate hydratase

Assay Type: SYBR® Green
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qRnoCID0001184
List Price:    $125.00
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PrimePCR™ Probe Assay: Aco1, Rat
Cytoplasmic aconitate hydratase

Assay Type: Probe
Assay Design: intron_spanning
Application: Gene Expression
Unique Assay ID: qRnoCIP0023015
List Price:    $180.00
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PrimePCR™ ddPCR™ Expression EvaGreen® Assay: Aco1, Rat
Cytoplasmic aconitate hydratase

Assay Type: EvaGreen
Application: Gene Expression
Unique Assay ID: dRnoEG5135716
Info:   EG; Same primer pair as used in qPCR assay qRnoCID0001184; intron_spanning
List Price:    $125.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Aco1, Rat
Cytoplasmic aconitate hydratase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5167410
Info:   FAM; Same primer pair and probe as used in qPCR assay qRnoCIP0023015; intron_spanning
 
Fluorophore:   FAM
List Price:    $190.00
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PrimePCR™ ddPCR™ Expression Probe Assay: Aco1, Rat
Cytoplasmic aconitate hydratase

Assay Type: Probe
Application: Gene Expression
Unique Assay ID: dRnoCPE5167411
Info:   HEX; Same primer pair and probe as used in qPCR assay qRnoCIP0023015; intron_spanning
 
Fluorophore:   HEX
List Price:    $190.00
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    Related pathways not available
Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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