Gene:  ACLY, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product acetyl-CoA serves several important biosynthetic pathways including lipogenesis and cholesterogenesis. In nervous tissue ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

PrimePCR™ SYBR® Green Assay: ACLY, Human
ATP citrate lyase

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0044387
Info:   Recommended - best coverage; Same primer pair as used in probe assay qHsaCEP0051649
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PrimePCR™ SYBR® Green Assay: ACLY, Human
ATP citrate lyase

Assay Type: SYBR® Green
Assay Design: Intron-spanning
Application: Gene Expression
Unique Assay ID: qHsaCID0015901
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PrimePCR™ Probe Assay: ACLY, Human
ATP citrate lyase

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0051649
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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