Gene:  ACACA, Human

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PrimePCR Primer Assays for Real-Time PCR oligo primer pair gene expression assay target

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Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA the rate-limiting step in fatty acid synthesis. There are two ACC forms alpha and beta encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene. [provided by RefSeq Jul 2008]

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PrimePCR™ SYBR® Green Assay: ACACA, Human
acetyl-CoA carboxylase alpha

Assay Type: SYBR® Green
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCED0038035
Info:   Same primer pair as used in probe assay qHsaCEP0040758
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PrimePCR™ Probe Assay: ACACA, Human
acetyl-CoA carboxylase alpha

Assay Type: Probe
Assay Design: exonic
Application: Gene Expression
Unique Assay ID: qHsaCEP0040758
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PrimePCR™ ddPCR™ Mutation Assay:ACACA p.R1629Q, Human
acetyl-CoA carboxylase alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505030
Info:   79nt amplicon; exonic; in-silico designed
 
Fluorophore:   FAM
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PrimePCR™ ddPCR™ Mutation Assay:ACACA WT for p.R1629Q, Human
acetyl-CoA carboxylase alpha

Assay Type: Probe
Application: In Silico Mutation Detection
Unique Assay ID: dHsaIS2505031
Info:   79nt amplicon; exonic; in-silico designed
 
Fluorophore:   HEX
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Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
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