PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000046189, Rat

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: No symbol available for ENSRNOG00000046189, Rat
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: No symbol available for ENSRNOG00000046189, Rat
Reaction:     4 billion copies/200 µl
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0050221
Assay Design:   Intron-spanning
Chromosome Location:   14:368215-369246question
Amplicon Length:   142
Splice Variants Targeted:   ENSRNOT00000042693 ENSRNOT00000073754 ENSRNOT00000034630 ENSRNOT00000044505 ENSRNOT00000074130 ENSRNOT00000072076 ENSRNOT00000074402 ENSRNOT00000071037 ENSRNOT00000043877 ENSRNOT00000070942 ENSRNOT00000052336 ENSRNOT00000050676 ENSRNOT00000049917 ENSRNOT00000045104 ENSRNOT00000049544 ENSRNOT00000039900 ENSRNOT00000052140 ENSRNOT00000072859 ENSRNOT00000071182 ENSRNOT00000072360 ENSRNOT00000073546 ENSRNOT00000045627 ENSRNOT00000041332 ENSRNOT00000050254 ENSRNOT00000044270 ENSRNOT00000049921 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000046189
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000046189
Entrez:   0
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 37.520000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download