PrimePCR™ Probe Assay: No symbol available for ENSRNOG00000048269, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0048736
Assay Design:   Intron-spanning
Chromosome Location:   1:205143706-205152664question
Amplicon Length:   68
Splice Variants Targeted:   ENSRNOT00000072324 ENSRNOT00000029985 ENSRNOT00000026413 ENSRNOT00000061446 ENSRNOT00000072065 ENSRNOT00000019072 ENSRNOT00000017005 ENSRNOT00000036876 ENSRNOT00000026195 ENSRNOT00000072788 ENSRNOT00000022022 ENSRNOT00000011855 ENSRNOT00000012222 ENSRNOT00000030244 ENSRNOT00000034264 ENSRNOT00000057830 ENSRNOT00000057218 ENSRNOT00000006752 ENSRNOT00000001536 ENSRNOT00000010753 ENSRNOT00000075110 ENSRNOT00000010229 ENSRNOT00000023226 ENSRNOT00000068334 ENSRNOT00000038612 ENSRNOT00000071526 ENSRNO

Gene Information

Description Not Available

Gene Symbol:   No symbol available for ENSRNOG00000048269
Gene Name:   Not Available
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSRNOG00000048269
Entrez:   0
UniGene:   Not Available
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.730000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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