PrimePCR™ Probe Assay: Scrt2, Rat

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RT PROBE IMG ALT TEXT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $190.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qRnoCIP0047691
Assay Design:   Intron-spanning
Chromosome Location:   3:153934827-153945839question
Amplicon Length:   187
Splice Variants Targeted:   ENSRNOT00000049759 ENSRNOT00000016452 ENSRNOT00000015467 ENSRNOT00000005690 ENSRNOT00000005700 ENSRNOT00000016380 ENSRNOT00000010895 ENSRNOT00000006833 ENSRNOT00000064159 ENSRNOT00000028271 ENSRNOT00000009178

Gene Information

Description Not Available

Gene Symbol:   Scrt2
Gene Name:   Transcriptional repressor scratch 2
Aliases:   Not Available
RefSeq:   NM_001191905
Ensembl:   ENSRNOG00000005148
Entrez:   366229
UniGene:   Rn.228224
Chromosome Mapping:   3q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Rat Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.890000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
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